Cell Culture:Article Title: IL‑1 receptor antagonism attenuates renal fibrosis via RNF182‑driven MFN2 destabilization and mitochondrial dysfunction.
Article Snippet: .. Cell Culture Human kidney proximal tubular epithelial cells (HK-2, ATCC CRL-2190) were cultured in DMEM/F-12 medium (Gibco, Cat# 11330032) supplemented with 10 % fetal bovine serum (FBS) (Gibco, Cat# 10270106) and 1 % penicillin-streptomycin (Gibco, Cat# 15140122). .. Rat kidney fibroblasts (NRK-49F, ATCC CRL-1570) AR TI CL E IN P RE SS had been cultured in DMEM high glucose (Gibco, Cat# 11965092) supplemented with 5 % FBS and 1 % penicillin-streptomycin.
Article Title: Inhibition of polyomavirus replication
Article Snippet: .. Cell Culture Human kidney proximal tubular epithelial cells (HK2, ATCC®) were maintained in Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (Gibco) supplemented with 3,3′,5-Triiodo-L-thyronine sodium salt (Sigma-Aldrich), insulin-transferrin-selenium-ethanolamine (ITS-X; Sigma-Aldrich), human epidermal growth factor (EGF: Sigma-Aldrich), hydrocortison (Sigma-Aldrich), and 100 U/mL penicillin-streptomycin (Gibco). .. Human renal proximal tubular epithelial cells (PTEC, Sciencell Research Laboratories) were maintained in complete REGMTM renal epithelial cell growth medium (Lonza).
Article Title: Inhibition of polyomavirus replication
Article Snippet: .. Cell Culture Human kidney proximal tubular epithelial cells (HK2, ATCC®) were maintained in Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (Gibco) supplemented with 3,3′,5-Triiodo-L-thyronine sodium salt (Sigma-Aldrich), insulin-transferrin-selenium-ethanolamine (ITS-X; Sigma-Aldrich), human epidermal growth factor (EGF; Sigma-Aldrich), hydrocortison (Sigma-Aldrich), and 100 U/mL penicillin-streptomycin (Gibco). .. Human renal proximal tubular epithelial cells (PTEC, Sciencell Research Laboratories) were maintained in complete REGMTM renal epithelial cell growth medium (Lonza).
Modification:Article Title: Inhibition of polyomavirus replication
Article Snippet: .. Cell Culture Human kidney proximal tubular epithelial cells (HK2, ATCC®) were maintained in Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (Gibco) supplemented with 3,3′,5-Triiodo-L-thyronine sodium salt (Sigma-Aldrich), insulin-transferrin-selenium-ethanolamine (ITS-X; Sigma-Aldrich), human epidermal growth factor (EGF: Sigma-Aldrich), hydrocortison (Sigma-Aldrich), and 100 U/mL penicillin-streptomycin (Gibco). .. Human renal proximal tubular epithelial cells (PTEC, Sciencell Research Laboratories) were maintained in complete REGMTM renal epithelial cell growth medium (Lonza).
Article Title: Inhibition of polyomavirus replication
Article Snippet: .. Cell Culture Human kidney proximal tubular epithelial cells (HK2, ATCC®) were maintained in Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (Gibco) supplemented with 3,3′,5-Triiodo-L-thyronine sodium salt (Sigma-Aldrich), insulin-transferrin-selenium-ethanolamine (ITS-X; Sigma-Aldrich), human epidermal growth factor (EGF; Sigma-Aldrich), hydrocortison (Sigma-Aldrich), and 100 U/mL penicillin-streptomycin (Gibco). .. Human renal proximal tubular epithelial cells (PTEC, Sciencell Research Laboratories) were maintained in complete REGMTM renal epithelial cell growth medium (Lonza).
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